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Gene tree discordance is expected in phylogenomic trees and biological processes are often invoked to explain it. However, heterogeneous levels of phylogenetic signal among individuals within datasets may cause artifactual sources of topological discordance. We examined how the information content in tips and subclades impacts topological discordance in the parrots (Order: Psittaciformes), a diverse and highly threatened clade of nearly 400 species. Using ultraconserved elements from 96% of the clade's species-level diversity, we estimated concatenated and species trees for 382 ingroup taxa. We found that discordance among tree topologies was most common at nodes dating between the late Miocene and Pliocene, and often at the taxonomic level of genus. Accordingly, we used two metrics to characterize information content in tips and assess the degree to which conflict between trees was being driven by lower quality samples. Most instances of topological conflict and non-monophyletic genera in the species tree could be objectively identified using these metrics. For subclades still discordant after tip-based filtering, we used a machine learning approach to determine whether phylogenetic signal or noise was the more important predictor of metrics supporting the alternative topologies. We found that when signal favored one of the topologies, noise was the most important variable in poorly performing models that favored the alternative topology. In sum, we show that artifactual sources of gene tree discordance, which are likely a common phenomenon in many datasets, can be distinguished from biological sources by quantifying the information content in each tip and modeling which factors support each topology. 

This symposium will focus on five projects’ professional development efforts to enhance educators’ understanding and use of the Next Generation Science Standards (NGSS). Involving educators from preschool to middle school levels in diverse contexts, each project has worked in this problem space in different ways. Of central importance to all the projects is how the NGSS necessitate productive classroom discourse, but the projects differ on how to support educators to achieve “rich science talk.” For example, an “assessment for learning” lens guides one group’s work, while recognizing language and argument as epistemic tools is the driving conceptual framework for another. In this symposium, project leaders discuss the decisions and dilemmas of, and the lessons learned from, their work. This highly interactive session includes brief introductions from each project followed by time for interaction with the projects’ researchers and materials. Projects will bring materials such as scaffolds for collaborative instructional planning, a formative classroom observation tool to support teachers’ use of productive classroom discourse, and examples of instructional units with 7 curricular features designed to support the vision of the NGSS. The session will culminate with time for crosstalk and discussion. 

Abstract DarkSide-20k is a novel liquid argon dark matter detector currently under construction at the Laboratori Nazionali del Gran Sasso (LNGS) of the Istituto Nazionale di Fisica Nucleare (INFN) that will push the sensitivity for Weakly Interacting Massive Particle (WIMP) detection into the neutrino fog. The core of the apparatus is a dual-phase Time Projection Chamber (TPC), filled with 50 tonnes of low radioactivity underground argon (UAr) acting as the WIMP target. NUV-HD-cryo Silicon Photomultipliers (SiPM)s designed by Fondazione Bruno Kessler (FBK) (Trento, Italy) were selected as the photon sensors covering two$$10.5~\text {m}^2$$ $10.5{\text{m}}^2$Optical Planes, one at each end of the TPC, and a total of$$5~\text {m}^2$$ $5{\text{m}}^2$photosensitive surface for the liquid argon veto detectors. This paper describes the Quality Assurance and Quality Control (QA/QC) plan and procedures accompanying the production of FBK NUV-HD-cryo SiPM wafers manufactured by LFoundry s.r.l. (Avezzano, AQ, Italy). SiPM characteristics are measured at 77 K at the wafer level with a custom-designed probe station. As of March 2025, 1314 of the 1400 production wafers (94% of the total) for DarkSide-20k were tested. The wafer yield is$$93.2\pm 2.5$$ $93.2\pm 2.5$%, which exceeds the 80% specification defined in the original DarkSide-20k production plan. 

Asymptotic giant branch stars are responsible for the production of most of the heavy isotopes beyond Sr observed in the solar system. Among them, isotopes shielded from the $r$-process contribution by their stable isobars are defined as $s$-only nuclei. For a long time the abundance of ${}^{204}\mathrm{Pb}$, the heaviest $s$-only isotope, has been a topic of debate because state-of-the-art stellar models appeared to systematically underestimate its solar abundance. Besides the impact of uncertainties from stellar models and galactic chemical evolution simulations, this discrepancy was further obscured by rather divergent theoretical estimates for the neutron capture cross section of its radioactive precursor in the neutron-capture flow, ${}^{204}\mathrm{Tl}$( $t_{1/2}=3.78\mathrm{yr}$), and by the lack of experimental data on this reaction. We present the first ever neutron capture measurement on ${}^{204}\mathrm{Tl}$, conducted at the CERN neutron time-of-flight facility n_TOF, employing a sample of only 9 mg of ${}^{204}\mathrm{Tl}$produced at the Institute Laue Langevin high flux reactor. By complementing our new results with semiempirical calculations we obtained, at the $s$-process temperatures of $kT\approx 8\mathrm{keV}$and $kT\approx 30\mathrm{keV}$, Maxwellian-averaged cross sections (MACS) of 580(168) mb and 260(90) mb, respectively. These figures are about 3% lower and 20% higher than the corresponding values widely used in astrophysical calculations, which were based only on theoretical calculations. By using the new ${}^{204}\mathrm{Tl}$MACS, the uncertainty arising from the ${}^{204}\mathrm{Tl}(\mathrm{n},\gamma )$cross section on the $s$-process abundance of ${}^{204}\mathrm{Pb}$has been reduced from $\sim 30\%$down to $+8\%/-6\%$, and the $s$-process calculations are in agreement with the latest solar system abundance of ${}^{204}\mathrm{Pb}$reported by K. Lodders in 2021. Published by the American Physical Society2024 

DarkSide-20k (DS-20k) is a dark matter detection experiment under construction at the Laboratori Nazionali del Gran Sasso (LNGS) in Italy. It utilises ∼ 100 t of low radioactivity argon from an underground source (UAr) in its inner detector, with half serving as target in a dual-phase time projection chamber (TPC). The UAr cryogenics system must maintain stable thermodynamic conditions throughout the experiment's lifetime of over 10 years. Continuous removal of impurities and radon from the UAr is essential for maximising signal yield and mitigating background. We are developing an efficient and powerful cryogenics system with a gas purification loop with a target circulation rate of 1000 slpm. Central to its design is a condenser operated with liquid nitrogen which is paired with a gas heat exchanger cascade, delivering a combined cooling power of more than 8 kW. Here we present the design choices in view of the DS-20k requirements, in particular the condenser's working principle and the cooling control, and we show test results obtained with a dedicated benchmarking platform at CERN and LNGS. We find that the thermal efficiency of the recirculation loop, defined in terms of nitrogen consumption per argon flow rate, is 95 % and the pressure in the test cryostat can be maintained within ±(0.1–0.2) mbar. We further detail a 5-day cool-down procedure of the test cryostat, maintaining a cooling rate typically within -2 K/h, as required for the DS-20k inner detector. Additionally, we assess the circuit's flow resistance, and the heat transfer capabilities of two heat exchanger geometries for argon phase change, used to provide gas for recirculation. We conclude by discussing how our findings influence the finalisation of the system design, including necessary modifications to meet requirements and ongoing testing activities. 

The dual-phase liquid argon time projection chamber is presently one of the leading technologies to search for dark matter particles with masses below 10 GeV c−2. This was demonstrated by the DarkSide-50 experiment with approximately 50 kg of low-radioactivity liquid argon as target material. The next generation experiment DarkSide-20k, currently under construction, will use 1,000 times more argon and is expected to start operation in 2027. Based on the DarkSide-50 experience, here we assess the DarkSide-20k sensitivity to models predicting light dark matter particles, including Weakly Interacting Massive Particles (WIMPs) and sub-GeV c−2 particles interacting with electrons in argon atoms. With one year of data, a sensitivity improvement to dark matter interaction cross-sections by at least one order of magnitude with respect to DarkSide-50 is expected for all these models. A sensitivity to WIMP–nucleon interaction cross-sections below 1 × 10−42 cm2 is achievable for WIMP masses above 800 MeV c−2. With 10 years exposure, the neutrino fog can be reached for WIMP masses around 5 GeV c−2.